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| Toxin Name |
κ-theraphotoxin-Ec2c |
| Source Species |
Eucratoscelus constrictus |
| Toxin Group |
Theraphotoxin |
| Description |
A non-phyla-specific neurotoxin with an inhibitory cystine knot fold. This neurotoxin potently blocks BKCa channels in cockroach dorsal unpaired median (DUM) neurons with an IC50 of 25 nM. This occurs in the absence of any shifts in the voltage dependence of activation. At concentrations up to 330 nM it lacks any actions on transient 'A-type' Kv, delayed-rectifier Kv, Nav, and both HVA and M-LVA Cav channels in cockroach DUM neurons. This toxin differs from the related toxins κ-TRTX-Ec2a and κ-TRTX-Ec2b in that it is toxic to mice by introcerebroventicular injection and is therefore not phyla-selective. |
| Discovered |
2008 |
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| This toxin last updated on Sep 08, 2010 |
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| Current Taxonomy |
Historic Taxonomy |
| Kingdom |
Animalia |
| Phylum |
Arthropoda |
| Class |
Arachnida |
| Order |
Araneae |
| Infra-order |
Mygalomorphae |
| Family |
Theraphosidae |
| Genus |
Eucratoscelus |
| Species |
constrictus |
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| "Eucratoscelus" "constrictus" |
| "Eucratoscelus" "longiceps" |
| "Harpactira" "constricta" |
| "Harpactira" "elevata" |
| "Pterinochilus" "constrictus" |
| "Pterinochilus" "spinifer" |
| "Pterinochilus" "vorax" |
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| Molecular Target |
ED50 |
IC50 |
Kd |
Pharmacophore |
Comment |
| Potassium channel, calcium-activated (Slo-type) (invertebrate) |
|
25.3
nM
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IC50 in dorsal unpaired medial neurons from the terminal abdominal ganglia of the adult American cockroach (Periplaneta americana) |
| Taxon |
ED50 |
LD50 |
PD50 |
Qualitative Information |
| Mus musculus |
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Injection of 25 pmol/g (1.8 µg/20 g mouse) resulted in strong neurotoxicity symptoms including convulsions, tonic paralysis, general ataxia, and respiratory paralysis
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| Gryllus bimaculatus |
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At a dose of ca. 1100 pmol/g (4 µg/g) this toxin causes rapid insecticidal activity, with complete paralysis within 5 min, and death within 15 min
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| Disulfide Bonds |
| Left Residue |
Right Residue |
Evidence |
| 2 |
16 |
By homology |
| 9 |
21 |
By homology |
| 15 |
25 |
By homology |
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| Peptide Sequences |
>as:κ-theraphotoxin-Ec2c Toxin from venom of the spider Eucratoscelus constrictus that blocks invertebrate large-conductance calcium-activated potassium channels
YCQFKMWTCDSERKCCEDMVCRLWCKLNL |
Full BLAST |
BLAST mature toxin only
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| Synonym |
Type |
| κ-theraphotoxin-Ec2c |
Recommended full name |
| κ-TRTX-Ec2c |
Recommended abbreviation |
| El2.1431 |
Synonym |
| ElTx2 |
Synonym (abbreviation) |
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