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	Toxin Name κ-theraphotoxin-Ec2a Source Species Eucratoscelus  constrictus Toxin Group Theraphotoxin Description An insect-selective neurotoxin with an inhibitory cystine knot fold. This insecticidal neurotoxin potently blocks BKCa channels in cockroach dorsal unpaired median (DUM) neurons with an IC50 of 3.7 nM. This occurs in the absence of any shifts in the voltage dependence of activation. At higher concentrations (330 nM) it partially inhibits cockroach delayed-rectifier Kv currents, but lacks any actions on transient 'A-type' Kv, Nav or both HVA and M-LVA Cav channels in cockroach DUM neurons. Closely related to the insect-selective neurotoxin κ-TRTX-Ec2b and the non phyla-selective neurotoxin κ-TRTX-Ec2c from the same spider. No toxicity in mice after intracerebroventricular injection of up to 25 pmol/g (1.8 µg/20 g mouse). Discovered 2008 This toxin last updated on Sep 08, 2010 Taxonomy Current Taxonomy Historic Taxonomy Kingdom Animalia Phylum Arthropoda Class Arachnida Order Araneae Infra-order Mygalomorphae Family Theraphosidae Genus Eucratoscelus Species constrictus "Eucratoscelus" "constrictus" "Eucratoscelus" "longiceps" "Harpactira" "constricta" "Harpactira" "elevata" "Pterinochilus" "constrictus" "Pterinochilus" "spinifer" "Pterinochilus" "vorax" Biological Activity Neurotoxin Molecular Target ED50 IC50 Kd Pharmacophore Comment Potassium channel, calcium-activated (Slo-type) (invertebrate) 3.7  nM IC50 in dorsal unpaired medial neurons from the terminal abdominal ganglia of the adult American cockroach (Periplaneta americana) Taxon ED50 LD50 PD50 Qualitative Information Gryllus bimaculatus At a dose of ca. 1100 pmol/g (4 µg/g) this toxin causes rapid insecticidal activity, with complete paralysis within 5 min, and death within 15 min Protein Information Disulfide Bonds Left Residue Right Residue Evidence 2 16 By homology 9 21 By homology 15 25 By homology Sequences Peptide Sequences >as:κ-theraphotoxin-Ec2a Toxin from venom of the spider Eucratoscelus constrictus that blocks invertebrate large-conductance calcium-activated potassium channels YCQKFLWTCDTERKCCEDMVCELWCKLEK Full BLAST BLAST mature toxin only Toxin Synonyms Synonym Type κ-theraphotoxin-Ec2a Recommended full name κ-TRTX-Ec2a Recommended abbreviation El2.1421 Synonym ElTx1 Synonym (abbreviation)
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