 |
|
 |
| |
| Toxin Name |
U2-agatoxin-Aop1a |
| Source Species |
Allagelena opulenta |
| Toxin Group |
Agatoxin |
| Description |
Insecticidal toxin causing rapid but reversible paralysis in crickets. No activity shown in mammals. Suppresses the excitatory post-synaptic potentials evoked in lobster neuromuscular synaptic preparations, possibly by blocking the presynaptic calcium channel. Does not bind to mammalian voltage-gated calcium channel subtypes Cav2.1, Cav2.2 or Cav2.3. The toxin sequence is identical to the insecticidal U2-agatoxin-Ao1a from the venom of the related species Agelena orientalis |
| Discovered |
1990 |
|
|
| This toxin last updated on Aug 20, 2010 |
|
| Current Taxonomy |
Historic Taxonomy |
| Kingdom |
Animalia |
| Phylum |
Arthropoda |
| Class |
Arachnida |
| Order |
Araneae |
| Infra-order |
Araneomorphae |
| Family |
Agelenidae |
| Genus |
Allagelena |
| Species |
opulenta |
|
| Agalena japonica |
| Agalena opulenta |
| Agelena japonica |
| Agelena opulenta |
| Allagelena opulenta |
|
|
| Molecular Target |
ED50 |
IC50 |
Kd |
Pharmacophore |
Comment |
| Molecular target unknown |
|
|
|
|
|
| Taxon |
ED50 |
LD50 |
PD50 |
Qualitative Information |
| Gryllus bimaculatus |
|
|
291.0
pmol/g
|
Synthesised toxin, by injection
|
|
| Original Deposition References |
Hagiwara K., Sakai T., Miwa A., Kawai N., Nakajima T.
Biomed. Res. 11:181-186 (1990)
Complete amino acid sequence of a new type of neurotoxin from the venom of the spider, Agelena opulenta.
|
Hagiwara K., Inui T., Nakajima K., Kimura T., Kitada C., Fujino M., Sakakibara S., Nakajima T.
Biomed. Res. 12:357-363 (1991)
Agelenin, a spider neurotoxin: determination of the C-terminus as amide form, and investigation of the disulfide bond arrangement.
|
Inui T., Hagiwara K., Nakajima K., Kimura T., Nakajima T., Sakakibara S.
Pept. Res. 5:140-144 (1992)
Synthesis and disulfide structure determination of agelenin: identification of the carboxy-terminus as an amide form.
|
| Other References |
Yamaji N, Sugase K., Nakajima T., Miki T., Wakamori M., Mori Y., Iwashita T.
FEBS Lett. 581:3789-3794 (2007)
Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors.
|
|
| Disulfide Bonds |
Posttranslational modifications |
| Left Residue |
Right Residue |
Evidence |
| 3 |
19 |
Experimentally determined |
| 10 |
24 |
Experimentally determined |
| 18 |
34 |
Experimentally determined |
|
| Residue Number |
Type |
Symbol |
| 35 |
C-terminal amidation |
NHâ‚‚ |
|
|
| Peptide Sequences |
>as:U1-agatoxin-Aop1a|sp:P31328 Neurotoxin peptide sequence, U1-agatoxin-Aop1a.
GGCLPHNRFCNALSGPRCCSGLKCKELSIWDSRCL |
Full BLAST |
BLAST mature toxin only
|
|
| Synonym |
Type |
| U1-agatoxin-Aop1a |
Recommended full name |
| U1-AGTX-Aop1a |
Recommended abbreviation |
| Agelenin |
Synonym |
|
|
|
|
|
 |
|
 |
|
|
